京都大学 植物病理学研究室
Laboratory of Plant Pathology, Kyoto University



Takagi M, Hirata R, Aihara Y, Hayashi Y, Aihara MM, Ando E, Kono MY, Tomiyama M, Kinoshita T, Mine A, Toda Y. Image-based quantification of Arabidopsis thaliana stomatal aperture from leaf images. (2023) Plant Cell Physiology, 20:pcad018.

Inoue Y, Phuong Vy TT, Singkaravanit-Ogawa S, Zhang R, Yamada K, Ogawa T, Ishizuka J, Narusaka Y, Takano Y. Selective deployment of virulence effectors correlates with host specificity in a fungal plant pathogen. (2023) New Phytologist, 238:1578-1592.

Takano Y, Inoue Y, Zhang R, Singkaravanit-Ogawa S, Matsuo H, Ogawa T, Jin C. Nonhost resistance and effectors in interactions between Colletotrichum species and plants. (2023) Physiological Molecular Plant Pathology, 125:1019820.

Yamada K, Yamamoto T, Uwasa K, Osakabe K, Takano Y. The establishment of multiple knockout mutants of Colletotrichum orbiculare by CRISPR-Cas9 and Cre-loxP systems. (2023) Fungal Genetics and Biology, 165:103777.


Takagi M, Hotamori K, Naito K, Matsukawa S, Egusa M, Nishizawa Y, Kanno Y, Seo M, Ifuku S, Mine A, Kaminaka H. Chitin-induced systemic disease resistance in rice requires both OsCERK1 and OsCEBiP and is mediated via perturbation of cell-wall biogenesis in leaves. (2022) Frontiers in Plant Science, 13:1064628.

Yoshihisa A, Yoshimura S, Shimizu M, Sato S, Matsuno S, Mine A, Yamaguchi K, Kawasaki T. The rice OsERF101 transcription factor regulates the NLR Xa1-mediated immunity induced by perception of TAL effectors. New Phytologist, 236:1441-1454.

Kato H, Nemoto K, Shimizu M, Abe A, Asai S, Ishihama N, Matsuoka S, Daimon T, Ojika M, Kawakita K, Onai K, Shirasu K, Yoshida M, Ishiura M, Takemoto D, Takano Y, Terauchi R. Recognition of pathogen-derived sphingolipids in Arabidopsis. (2022) Science, 376:857-860.

Saroopa P. Samaradivakara, Chen H, Lu Y, Li P, Kim Y, Tsuda K, Mine A, Day B. Overexpression of NDR1 leads to pathogen resistance at elevated temperatures. (2022) New Phytologist, 235:1146–1162.

Takata S, Mise K, Takano Y, Kaido M. Subcellular dynamics of red clover necrotic mosaic virus double-stranded RNAs in infected plant cells. (2022) Virology, 568:126-139.


Tominaga T, Miura C, Sumigawa Y, Hirose Y, Yamaguchi K, Shigenobu S, Mine A, Kaminaka H. Conservation and Diversity in Gibberellin-Mediated Transcriptional Responses Among Host Plants Forming Distinct Arbuscular Mycorrhizal Morphotypes. (2021) Frontiers in Plant Science, 12:795695.

Zhang R, Isozumi N, Mori M, Okuta R, Singkaravanit-Ogawa S, Imamura T, Kurita JI, Gan P, Shirasu K, Ohki S, Takano Y.  Fungal effector SIB1 of Colletotrichum orbiculare has unique structural features and can suppress plant immunity in Nicotiana benthamiana. (2021) Journal of Biological Chemistry, 297:101370.

Singkaravanit-Ogawa S, Kosaka A, Kitakura S, Uchida K, Nishiuchi T, Ono E, Fukunaga S, Takano Y. Arabidopsis CURLY LEAF functions in leaf immunity against fungal pathogens by concomitantly repressing SEPALLATA3 and activating ORA59. (2021) The Plant Journal, 108:1005-1019.

Tsushima A, Narusaka M, Gan P, Kumakura N, Hiroyama R, Kato N, Takahashi S, Takano Y, Narusaka Y, Shirasu K. The Conserved Colletotrichum spp. effector candidate CEC3 induces nuclear expansion and cell death in plants. (2021) Frontiers in Microbiology, 12:682155

Matsumoto A, Schlüter T, Melkonian K, Takeda A, Nakagami H, Mine A. A versatile Tn7 transposon-based bioluminescence tagging tool for quantitative and spatial detection of bacteria in plants. (2021) Plant Communications, 3:100227.

Iwakawa HO, Lam AYW, Mine A, Fujita T, Kiyokawa K, Yoshikawa M, Takeda A, Iwasaki S, Tomari Y. Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants. (2021) Cell Reports, 35:109300

Chen J, Inoue Y, Kumakura N, Mise K, Shirasu K, Takano Y. Comparative transient expression analyses on two conserved effectors of Colletotrichum orbiculare reveal their distinct cell death-inducing activities between Nicotiana benthamiana and melon. (2021) Mol Plant Pathology, 22:1006-1013.

Ogawa T, Chen J, Mise K, Takano Y. Multiple Colletotrichum species commonly exhibit focal effector accumulation in a biotrophic interface at the primary invasion sites in their host plants. (2021) Plant Signaling & Behavior, 16:1935604.

Irieda H, Takano Y. Epidermal chloroplasts are defense-related motile organelles equipped with plant immune components. (2021) Nature Communications, 12:2739

Fujisaki K, Tateda C, Abe Y, Dominguez JJA, Iwai M, Obara K, Nakamura T, Iwadate Y, Kaido M, and Mise K. (2021). Infectious in vitro transcripts from a cDNA clone of a Japanese gentian isolate of Sikte waterborne virus, which shows host-specific low-temperature-dependent replication. Archives of Virology, 166:1991-1997.

Gan P, Hiroyama R, Tsushima A, Masuda S, Shibata A, Ueno A, Kumakura N, Narusaka M, Hoat TX, Narusaka Y, Takano Y, Shirasu K. Telomeres and a repeat-rich chromosome encode effector gene clusters in plant pathogenic Colletotrichum fungi. (2021) Environ Microbiol, 23:6004-6018.

Kosaka A, Pastorczyk M, Piślewska-Bednarek M, Nishiuchi T, Ono E, Suemoto H, Ishikawa A, Frerigmann H, Kaido M, Mise K, Bednarek P, Takano Y. Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola. (2021) Scientific Reports, 11:1488.


Fujisaki K, Abe Y, Tateda C, Iwai M, Kaido M, and Mise K. Host specific preference for low temperature in the multiplication of a tombusvirus, gentian virus A. (2020) Virus Research, 286:198048.

Kosaka A, Suemoto H, Singkaravanit-Ogawa S, and Takano Y. Plant defensin expression triggered by fungal pathogen invasion depends on EDR1 protein kinase and ORA59 transcription factor in Arabidopsis thaliana. (2020) Plant Signaling & Behavior, 28:1823120.

Kato H, Onai K, Abe A, Shimizu M, Takagi H, Tateda C, Utsushi H, Singkarabanit-Ogawa S, Kitakura S, Ono E, Zipfel C, Takano Y, Ishiura M, and Terauchi R. Lumi-Map, a real-time luciferase bioluminescence screen of mutants combined with MutMap, reveals Arabidopsis genes involved in PAMP-triggered immunity. (2020) Molecular Plant-Microbe Interactions, 33:1366-1380.

Ono E, Mise K, and Takano Y. RLP23 is required for Arabidopsis immunity against the grey mould pathogen Botrytis cinerea. (2020) Scientic Reports, 10:13798.


Shimada TL, Shimada T, Okazaki Y, Higashi Y, Saito K, Kuwata K, Oyama K, Kato M, Ueda H, Nakano A, Ueda T, Takano Y, and Hara-Nishimura I. HIGH STEROL ESTER 1 is a key factor in plant sterol homeostasis. (2019) Nature Plants, 5:1154-1166.

Pastorczyk M, Kosaka A, Piślewska-Bednarek M, López G, Frerigmann H, Kułak K, Glawischnig E, Molina A, Takano Y, and Bednarek P. The role of CYP71A12 monooxygenase in pathogen-triggered tryptophan metabolism and Arabidopsis immunity. (2019) New Phytologist, 225:400-412.

Gan P, Tsushima A, Hiroyama R, Narusaka M, Takano Y, Narusaka Y, Kawaradani M, Damm U, and Shirasu K. Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence. (2019) Scientific Reports 9:13349.

Isozumi N, Inoue Y, Imamura T, Mori M, Takano Y, and Ohki S. Ca2+-dependent interaction between calmodulin and CoDN3, an effector of Colletotrichum orbiculare. (2019) Biochemical and Biophysical Research Communications , 514:803-808.

Tsushima A, Gan P, Kumakura N, Narusaka M, Takano Y, Narusaka Y, and Shirasu K. Genomic plasticity mediated by transposable elements in the plant pathogenic fungus Colletotrichum higginsianum. (2019) Genome Biology and Evolution, 11:1487-1500.

Shimada TL, Betsuyaku S, Inada N, Ebine K, Fujimoto M, Uemura T, Takano Y, Fukuda H, Nakano A, and Ueda T. Enrichment of phosphatidylinositol 4,5-bisphosphate in the extra-invasive hyphal membrane promotes Colletotrichum infection of Arabidopsis thaliana. (2019) Plant Cell Physiology, 60:1514-1524.

Gan P, Tsushima A, Narusaka M, Narusaka Y, Takano Y, Kubo Y, and Shirasu K. Genome sequence resources for four phytopathogenic fungi from the Colletotrichum orbiculare species complex. (2019) Molecular Plant-Microbe Interactions, 32:1088-1090.

Hamada T, Mise K, Kiba A, and Hikichi Y. Systemic necrosis in tomato induced by a Japanese isolate of rehmannia mosaic virus in a temperature sensitive manner. (2019) Plant Pathology 68:1025-1032.

Kaido M, Nagano H, Omote K, Mise K, Okuno T, and Takano Y. 5’-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs. (2019) Virus Research 265:138-142.

Irieda, H., Inoue, Y., Mori, M., Yamada , K., Oshikawa, Y., Saitoh, H., Uemura, A., Terauchi, R., Kitakura, S., Kosaka, K., Singkaravanit-Ogawa, S., and Takano, Y. Conserved fungal effector suppresses PAMP-triggered immunity by targeting plant immune kinases. (2019) Proc. Natl. Acad. Sci. U.S.A. 116:496-505.


Kosaka, A., and Takano, Y. Nonhost resistance of Arabidopsis thaliana against Colletotrichum species. (2018) Journal of General Plant Pathology 84:305-311.

Piślewska-Bednarek M, Nakano RT, Hiruma K, Pastorczyk M, Sánchez-Vallet A, Singkaravanit-Ogawa S, Ciesiołka D, Takano Y, Molina A, Schulze-Lefert P, and Bednarek P. Glutathione transferase U13 functions in pathogen-triggered glucosinolate metabolism. (2018) Plant Physiology 176:538-551.

Ahmad Azmi NS, Singkaravanit-Ogawa S, Ikeda K, Kitakura S, Inoue Y, Narusaka Y, Shirasu K, Kaido M, Mise K, and Takano Y. Inappropriate expression of an NLP effector in Colletotrichum orbiculare impairs infection on Cucurbitaceae cultivars via plant recognition of the C-terminal region. (2018) Molecular Plant-Microbe Interactions 31:101-111.


Tajima Y, Iwakawa HO, Hyodo K, Kaido M, Mise K, and Okuno T. Requirement for eukaryotic translation initiation factors in cap-independent translation differs between bipartite genomic RNAs of red clover necrotic mosaic virus. (2017) Virology 509: 152-158.

Hyodo K, Suzuki N, Mise K, and Okuno T. Roles of superoxide anion and hydrogen peroxide during replication of two unrelated plant RNA viruses in Nicotiana benthamiana. (2017) Plant Signaling & Behavior 8: e1338223.

Gan P, Narusaka M, Tsushima A, Narusaka Y, Takano Y, and Shirasu K. Draft genome assembly of Colletotrichum chlorophyti, a pathogen of herbaceous plants.(2017) Genome Announcements 5: e01733-16.

Fukunaga S, Sogame M, Hata M, Singkaravanit-Ogawa S, Piślewska-Bednarek M, Onozawa-Komori M, Nishiuchi T, Hiruma K, Saitoh H, Terauchi R, Kitakura S, Inoue Y, Bednarek P, Schulze-Lefert P, and Takano Y. Dysfunction of Arabidopsis MACPF domain protein activates programmed cell death via tryptophan metabolism in MAMP-triggered immunity. (2017) Plant Journal 89: 381-393.


Yamada K, Saijo Y, Nakagami H, and Takano Y. Regulation of sugar transporter activity for antibacterial defense in Arabidopsis. (2016) Science 354: 1427-1430.

Yasui Y, Hirakawa H, Oikawa T, Toyoshima M, Matsuzaki C, Ueno M, Mizuno N, Nagatoshi Y, Imamura T, Miyago M, Tanaka K, Mise K, Tanaka T, Mizukoshi H, Mori M, and Fujita Y. Draft genome sequence of an inbred line of Chenopodium quinoa, an allotetraploid crop with great environmental adaptability and outstanding nutritional properties. (2016) DNA Research 23: 535-546.

Inada N, Betsuyaku S, Shimada TL, Ebine K, Ito E, Kutsuna N, Hasezawa S, Takano Y, Fukuda H, Nakano A, and Ueda T. Modulation of plant RAB GTPase-mediated membrane trafficking pathway at the interface between plants and obligate biotrophic pathogens. (2016) Plant Cell Physiology 57:1 854-1864.

Gan P, Narusaka M, Kumakura N, Tsushima A, Takano Y, Narusaka Y, and Shirasu K. Genus-wide comparative genome analyses of Colletotrichum species reveal specific gene family losses and gains during adaptation to specific infection lifestyles. (2016) Genome Biology and Evolution 8: 1467-1481.

Irieda H, and Takano Y. Identification and characterization of virulence-related effectors in the cucumber anthracnose fungus Colletotrichum orbiculare.(2016) Physiological and Molecular Plant Pathology 95: 87-92.

Irieda H, Ogawa S, and Takano Y. Focal effector accumulation in a biotrophic interface at the primary invasion sites of Colletotrichum orbiculare in multiple susceptible plants. (2016) Plant Signaling & Behavior 11: e1137407.

Narusaka M, Toyoda K, Shiraishi T, Iuchi S, Takano Y, Shirasu K, and Narusaka Y. Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. (2016) Scientific Reports 6: 18702.


Shimada TL, Takano Y, and Hara-Nishimura I. Oil body-mediated defense against fungi: From tissues to ecology. (2015) Plant Signaling & Behavior 10: e989036.

Hyodo K, Taniguchi T, Manabe Y, Kaido M, Mise K, Sugawara T, Taniguchi H, and Okuno T. Phosphatidic acid produced by phospholipase D promotes RNA replication of a plant RNA virus. (2015) PLoS Pathogens 11: e1004909.


Kaido M, Abe K, Mine A, Hyodo K, Taniguchi T, Taniguchi H, Mise K and Okuno T. GAPDH-A recruites a plant virus movement protein to cortical virus replication complexes to facilitate viral cell-to-cell movement. (2014) PLOS Pathogens e1004505.

Mizumoto H, Morikawa Y, Ishibashi K, Kimura K, Matsumoto K, Tokunaga M, Kiba A, Ishikawa M, Okuno T and Hikichi Y. Functional characterization of the mutations in Pepper mild mottle virus overcoming tomato tm1-mediated resistance. (2014) Molecular Plant Pathology 15: 479-487.

Hyodo K, Kaido M, and Okuno T. Traffic jam on the cellular secretory pathway generated by a replication protein from a plant virus. (2014) Plant Signaling & Behavior 9. pii: e28644.

Oku, M., Takano, Y., and Sakai, Y. The emerging role of autophagy in peroxisome dynamics and lipid metabolism of phyllosphere microorganisms. (2014) Frontiers in Plant Science 5 : article 81.

Narabayashi T, Kaido M, Okuno T, and Mise K. Base-paired structure in the 5′ untranslated region is required for the efficient amplification of negative-strand RNA3 in the bromovirus melandrium yellow fleck virus. (2014) Virus Research 188: 162-169.

Irieda H, Maeda H, Akiyama K, Saitoh H, Uemura A, Terauchi R, and Takano Y. Colletotrichum orbiculare secrets virulence effectors to a biotrophic interface at the primary hyphal neck via exocytosis coupled with SEC22-mediated traffic. (2014) Plant Cell 26: 2265-2281.

Ihara M, Takano Y, and Yamashita A. General flexible nature of the cytosolic regions of fungal transient receptor potential channels, revealed by expression screening using GFP-fusion techniques. Protein Science, 23: 923-931. (2014).

Saitoh H, Hirabuchi A, Fujisawa S, Mitsuoka C, Terauchi R, and Takano Y. MoST1encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae. FEMS Microbiology Letters, 352: 104-113. (2014).

Ross A, Yamada K, Hiruma K, Yamashita-Yamada M, Lu X, Takano Y, Tsuda K, and Saijo Y. The Arabidopsis PEPR pathway couples local and systemic plant immunity. (2014) EMBO Journal, 33: 62-75.

Shimada TL, Takano Y, Shimada T, Fujiwara M, Fukao Y, Mori M, Okazaki Y, Saito K, Sasaki R, Aoki K, and Hara-Nishimura I. Leaf oil body functions as a subcellular factory for the production of a phytoalexin in Arabidopsis. (2014) Plant Physiology 164: 105-118.

Hyodo K. and Okuno T. Host factors used by positive-strand RNA plant viruses for genome replication. (2014) Journal of General Plant Pathology 80: 123-135.


Hyodo K, Mine A, Taniguchi T, Kaido M, Mise K, Taniguchi H, and Okuno T. The ADP-ribosylation factor 1 plays an essential role in the replication of a plant RNA virus. (2013) Journal of Virology 87: 163-176.

Watanabe S, Shimada TL, Hiruma K, Takano Y. Watanabe S, Shimada TL, Hiruma K, and Takano Y. Pathogen infection trial increases the secretion of proteins localized in the endoplasmic reticulum body of Arabidopsis. (2013) Plant Physiology 163: 659-664.

Hiruma K, Fukunaga S, Bednarek P, Pislewska-Bednarek M, Watanabe S, Narusaka Y, Shirasu K, and Takano Y. Glutathione and tryptophan metabolism are required for Arabidopsis immunity during the hypersensitive response to hemibiotrophs. (2013) Proc. Natl. Acad. Sci. USA 110: 9589-9594.

Narusaka M, Kubo Y, Hatakeyama K, Imamura J, Ezura H, Nanasato Y, Tabei Y, Takano Y, Shirasu K, and Narusaka Y. Breaking restricted taxonomic functionality by dual resistance genes. (2013) Plant Signaling & Behavior 8: e24244.

Narusaka M, Kubo Y, Hatakeyama K, Imamura J, Ezura H, Nanasato Y, Tabei Y, Takano Y, Shirasu K, and Narusaka Y. Interfamily transfer of dual NB-LRR genes confers resistance to multiple pathogens. (2013) PLoS One 8: e55954.

Egusa M, Miwa T, Kaminaka H, Takano Y, and Kodama M. Nonhost resistance of Arabidopsis thaliana against Alternaria alternata involves both pre- and postinvasive defenses but is collapsed by AAL-toxin in the absence of LOH2. (2013) Phytopathology 103: 733-740.

Gan P, Ikeda K, Irieda H, Narusaka M, O’Connell RJ, Narusaka Y, Takano Y, Kubo Y, and Shirasu K. Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi. (2013) New Phytologist 197: 1236-1249.

Kubo Y, and Takano Y. Dynamics of infection-related morphogenesis and pathogenesis in Colletotrichum orbiculare. (2013) Journal of General Plant Pathology 79: 233-242.

Okuno T, and Hiruki C. Molecular biology and epidemiology of dianthoviruses. (2013) Advances in Virus Research 87: 37-74.

Hiruma K, Fukunaga S, Bednarek P, and Takano Y. Glutathione and tryptophan metabolites are key players in Arabidopsis nonhost resistance against Colletotrichum gloeosporioides. (2013) Plant Signaling & Behavior 8: e25603.


Mine A, Hyodo K, Tajima Y, Kusumanegara K, Taniguchi T, Kaido M, Mise K. Taniguchi H, and Okuno T. Differential roles of Hsp70 and Hsp90 in the assembly of the replicase complex of a positive strand RNA plant virus. (2012) Journal of Virology 86: 12091-12104.

Kusumanegara K, Mine A, Hyodo K, Kaido M, Mise K, and Okuno T. Identification of domains in p27 auxiliary replicase protein essential for its association with the endoplasmic reticulum membranes in Red clover necrotic mosaic virus. (2012) Virology 433: 131-141.

Iwakawa H-O, Tajima Y, Taniguchi T, Kaido M, Mise K, Tomari Y, Taniguchi H, and Okuno T. Poly(A)-binding protein facilitates translation of an uncapped/non-polyadenylated viral RNA by binding to the 3′ untranslated region. (2012) Journal of Virology 86: 7836-7849.

Asakura M, Yoshino K, Hill AM, Kubo Y, Sakai Y, and Takano Y. Primary and secondary metabolism regulates lipolysis in appressoria of Colletotrichum orbiculare. (2012) Fungal Genetics and Biology 49: 967-975.

Lin SY, Okuda S, Ikeda K, Okuno T, and Takano Y. LAC2 encoding a secreted laccase is involved in appressorial melanization and conidial pigmentation in Colletotrichum orbiculare. (2012) Molecular Plant-Microbe Interactions 25: 1552-1561.

O’Connell RJ, Thon MR, Hacquard S, Amyotte SG, Kleemann J, Torres MF, Damm U, Buiate EA, Epstein L, Alkan N, Altmuller J, Alvarado-Balderrama L, Bauser CA, Becker C, Birren BW, Chen Z, Choi J, Crouch JA, Duvick JP, Farman MA, Gan P, Heiman D, Henrissat B, Howard RJ, Kabbage M, Koch C, Kracher B, Kubo Y, Law AD, Lebrun MH, Lee YH, Miyara I, Moore N, Neumann U, Nordstrom K, Panaccione DG, Panstruga R, Place M, Proctor RH, Prusky D, Rech G, Reinhardt R, Rollins JA, Rounsley S, Schardl CL, Schwartz DC, Shenoy N, Shirasu K, Sikhakolli UR, Stuber K, Sukno SA, Sweigard JA, Takano Y, Takahara H, Trail F, van der Does HC, Voll LM, Will I, Young S, Zeng Q, Zhang J, Zhou S, Dickman MB, Schulze-Lefert P, Ver Loren van Themaat E, Ma LJ, and Vaillancourt LJ. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. (2012) Nature Genetics 44: 1060-5.

Saitoh H, Fujisawa S, Mitsuoka C, Ito A, Hirabuchi A, Ikeda K, Irieda H, Yoshino K, Yoshida K, Matsumura H, Tosa Y, Win J, Kamoun S, Takano Y, Terauchi R. Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a secreted protein required for infection by monocot and dicot fungal pathogens. (2012) PLOS Pathogens 8: e1002711.

Yoshino K, Irieda H, Sugimoto F, Yoshioka H, Okuno T, and Takano Y. Cell death of Nicotiana benthamiana is induced by secreted protein NIS1 of Colletotrichum orbiculare and is suppressed by a homologue of CgDN3. (2012) Molecular Plant-Microbe Interactions 25: 625-636.


Tajima Y, Iwakawa H-O, Kaido M, Mise K, and Okuno T. A long-distance RNA-RNA interaction plays an important role in programmed -1 ribosomal frameshifting in the translation of p88 replicase protein of Red clover necrotic mosaic virus. (2011) Virology 417: 169-178.

Hyodo K, Mine A, Iwakawa H-O, Kaido M, Mise K, and Okuno T. Identification of amino acids in auxiliary replicase protein p27 critical for its RNA-binding activity and the assembly of the replicase complex in Red clover necrotic mosaic virus. (2011) Virology 413: 300-309.

Kaido M, Funatsu N, Tsuno Y, Mise K, and Okuno T. Viral cell-to-cell movement requires formation of cortical punctate structures containing Red clover necrotic mosaic virus movement protein. (2011) Virology 413: 205-215.

Iwakawa H-O, Mine A, Hyodo K, An M, Kaido M, Mise K, and Okuno T. Template recognition mechanism by replicase proteins differ between bipartite positive-strand genomic RNAs of a plant virus. (2011) Journal of Virology 85: 497-509.

Hiruma K, Nishiuchi T, Kato T, Bednarek P, Okuno T, Schulze-Lefert P, and Takano Y. Arabidopsis ENHANCED DISEASE RESISTANCE 1 is required for pathogen-induced expression of plant defensins in nonhost resistance, and acts through interference of MYC2-mediated repressor function. (2011) Plant Journal 67: 980-992.

Hiruma K, and Takano Y. Roles of EDR1 in non-host resistance of Arabidopsis.  (2011) Plant Signaling & Behavior 6: 1831-1833.


Mine A, Hyodo K, Takeda A, Kaido M, Mise K, and Okuno T. Interactions between p27 and p88 replicase proteins of Red clover necrotic mosaic virus play an essential role in viral RNA replication and suppression of RNA silencing via the 480-kDa viral replicase complex assembly. (2010) Virology 407: 213-224.

Hiruma K, Onozawa-Komori M, Takahashi F, Asakura M, Bednarek P, Okuno T, Schulze-Lefert P, and Takano, Y. Entry mode-dependent function of an indole glucosinolate pathway in Arabidopsis for nonhost resistance against anthracnose pathogens. (2010) Plant Cell 22: 2429-2443.

An M, Iwakawa H, Mine A, Kaido M, Mise K, and Okuno, T. A Y-shaped RNA structure in the 3’untranslated region together with the trans-activator and core promoter of Red clover necrotic mosaic virus RNA2 is required for its negative-strand RNA synthesis. (2010) Virology 405: 100-109.

Mine A, Takeda A, Taniguchi T, Taniguchi H, Kaido M, Mise K, and Okuno T. Identification and characterization of the 480 kDa template-specific RNA-dependent RNA polymerase complex of Red clover necrotic mosaic virus. (2010) Journal of Virology 84: 6070-6081.

Yoshimoto K, Takano Y, and Sakai Y. Autopahgy in plants and phytopathogens. (2010) FEBS Letters 584:1350-1358.


Asakura M, Ninomiya S, Sugimoto M, Oku M, Yamashita S, Okuno T, Sakai Y, and Takano Y. Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare. (2009) Plant Cell 21:1291-1304.

Kaido M, Tsuno Y, Mise K, and Okuno T. Endoplasmic reticulum targeting of the Red clover necrotic mosaic virus movement protein is associated with the replication of viral RNA1 but not RNA2. (2009) Virology 395: 232-242.

Narabayashi T, Iwahashi F, Kaido M, Okuno T, and Mise K. Melandrium yellow fleckbromovirus infects Arabidopsis thaliana and has genomic RNA sequence characteristics that are unique among bromoviruses. (2009) Archives of Virology 154: 1381-1389.

Yoshida K, Saitoh H, Fujisawa S, Kanzaki H, Matsumura H, Yoshida K, Tosa Y, Chuma I, Takano Y, Win J, Kamoun S, and Terauchi R. Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae. (2009) Plant Cell 21:1573-1591.

Saitoh H, Fujisawa S, Ito A, Mitsuoka C, Berberich T, Tosa Y, Asakura M, Takano Y, and Terauchi R. SPM1 encoding a vacuole-localized protease is required for infection-related autophagy of the rice blast fungus Magnaporthe oryzae. (2009) FEMS Microbiology Letters 300: 115-121.

Sarawaneeyaruk S, Iwakawa H, Mizumoto H, Murakami H, Kaido M, Mise K, and Okuno T. Host-dependent roles of the viral 5’untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3′ UTR of Red clover necrotic mosaic virus RNA1. (2009) Virology 391: 107-118.

Fujisaki K, Iwahashi F, Kaido M, Okuno T, and Mise K. Genetic analysis of a host determination mechanism of bromoviruses in Arabidopsis thaliana. (2009) Virus Research 140: 103-110.

Takano Y, Asakura M, and Sakai Y. Atg26-mediated pexophagy and fungal phytopathogenicity. (2009) Autophagy 5: 1041-1042.

Asakura M, and Takano Y. Peroxisomes and phytopathogenicity. Terlecky, S.-R. and Titorenko, V.-I.(eds.) (2009) In Emergent Functions of the Peroxisome 34-42.

Terauchi R, Win J, Kamoun S, Matsumura H, Saitoh H, Yoshida K, Fujisawa S, Shenton M, Berberich T, Ito A, Takano Y, and Tosa, Y. A multi-faceted genomics approach toward understanding Magnaporthe-rice interactions. Lorito, M. (ed.) (2009) In Biology of Molecular Plant-Microbe Interactions Vol. 6.

Terauchi R, Win J, Kamoun S, Matsumura H, Saitoh H, Kanzaki H, Yoshida K, Shenton M, Berberich T, Fujisawa S, Ito A, Takano Y, and Tosa Y. Searching for effectors of Magnaporthe oryzae: a multi-faceted genomics approach. Wang, G.-L. and Pang (eds.) (2009) In Rice Blast 105-112.


Iwakawa H, Mizumoto H, Nagano H, Imoto Y, Takigawa K, Sarawaneeyaruk S, Kaido M, Mise K,and Okuno T. A viral non-coding RNA generated by cis-element-mediated protection against 5’→3′ RNA decay represses both cap-independent and cap-dependent translation. (2008) Journal of Virology 82: 10162-10174.

Matsumoto K, Tomikawa C, Toyooka T, Ochi A, Takano Y, Takayanagi N, Endo Y, and Hori H. Production of yeast tRNA (m7G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. (2008) Journal of Biotechnology 133:453-460.

Okamoto K, Nagano H, Iwakawa H, Mizumoto H, Takeda A, Kaido M, Mise K, and Okuno T. cis-Preferential requirement of a- 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1. (2008) Virology 375:205-212.

Matsumoto K, Sawada H, Matsumoto H, Hamada H, Yoshimoto E, Ito T, Takeuchi S, Tsuda S, Suzuki K, Kobayashi K, Kiba A, Okuno T,and Hikichi Y. The coat protein gene of tobamovirus P0 pathotype is a determinant for activation of temperature-insensitive L1a-gene-mediated resistance in Capsicum plants. (2008) Archives of Virology 153: 645-650.


Akamatsu N, Takeda A, Kishimoto M, Kaido M, Okuno T, and Mise K. Phosphorylation and interaction of the movement and coat proteins of brome mosaic virus in infected barley protoplasts. (2007) Archives of Virology 152: 2087-2093.

Iwakawa H, Kaido M, Mise K, and Okuno T. cis-Acting core RNA elements required for negative-strand RNA synthesis and cap-independent translation are separated in the 3′-untranslated region of Red clover necrotic mosaic virus RNA1. (2007) Virology 369: 168-181.

Kaido M, Inoue Y, Takeda Y, Sugiyama K, Takeda A, Mori M, Tamai A, Meshi T, Okuno T. and Mise K. Downregulation of the NbNACa1 gene encoding a movement-protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana. (2007) Molecular Plant-Microbe Interactions 20: 671-681.

Kudo C, Suzuki T, Fukuoka S, Asai S, Suenaga H, Sasabe M, Takano Y, Okuno T, Toyoda K, Shiraish T, Ichinose Y, and Inagaki Y. Suppression of Cdc27B expression induces plant defence responses. (2007) Molecular Plant Pathology 8: 365-373.

Matsumoto K, Toyooka T, Tomikawa C, Ochi A, Takano Y, Takayanagi N, Endo Y. and Hori, H. RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex). (2007) FEBS Letters 581: 1599-1604.


Asakura M, Okuno T, and Takano Y. Multiple contributions of peroxisomal metabolic function to fungal pathogenicity in Colletotrichum lagenarium. (2006) Applied and Environmental Microbiology 72:6345-6354.

Takano Y, Takayanagi N, Hori H, Ikeuchi Y, Suzuki T, Kimura A,and Okuno T. A gene involved in modifying transfer RNA is required for fungal pathogenicity and stress tolerance of Colletotrichum lagenarium. (2006) Molecular Microbiology 60: 81-92.

Mizumoto H, Iwakawa H-O, Kaido M, Mise K,and Okuno T. Cap-independent translation mechanism of Red clover necrotic mosaic virus RNA2 differs from that of RNA1 and is linked to RNA replication. (2006) Journal of Virology 80: 3781-3791.

Shimada C, Lipka V, O’Connell R, Okuno T, Schulze-Lefert P,and Takano Y. Nonhost resistance in Arabidopsis-Colletotrichum interactions acts at the cell periphery and requires actin filament function. (2006) Molecular Plant-Microbe Interactions 19: 270-279.

Watanabe T, Takeda A, Tsukiyama T, Mise K, Okuno T, Sasaki H, Minami N,and Imai H. Identification and characterization of two novel classes small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. (2006) Genes & Development 20: 1732-1743.

Okuno T. and Mizumoto H. Replication of Red clover necrotic mosaic virus. (2006) In Recent Advances in RNA Virus Replication (ed. K. H. Hefferon) Transworld Research Network,


Takeda A, Tsukuda M, Mizumoto H, Okamoto K, Kaido M, Mise K, and Okuno T. A plant RNA virus suppresses RNA silencing through viral RNA replication. (2005) EMBO Journal 24: 3147-3157.

Iwahashi F, Fujisaki K, Kaido M, Okuno T,and Mise K. Synthesis of infectious in vitro transcripts from Cassia yellow blotch bromovirus cDNA clones and a reassortment analysis with other bromoviruses in protoplasts. (2005) Archives of Virology 150: 1301-1314.

Sasaki N, Kaido M, Okuno T, and Mise K. Coat protein-independent cell-to-cell movement of bromoviruses expressing brome mosaic virus movement protein with an adaptation-related amino acid change in the central region. (2005) Archives of Virology 150: 1231-1240.

Takeda A, Nakamura W, Sasaki N, Goto K, Kaido M, Okuno T,and Mise K. Natural isolates of Brome mosaic virus with the ability to move from cell to cell independently of coat protein. (2005) Journal of General Virology 86: 1201-1211.

Tatsuta M, Mizumoto H, Kaido M, Mise K,and Okuno T. The Red clover necrotic mosaic virus RNA2 trans-activator is also a cis-acting RNA2 replication element. (2005) Journal of Virology 79: 978-986.

Yoshimi A, Kojima K, Takano Y,and Tanaka C. Group III histidine kinase is a positive regulator of Hog1-type mitogen-activated protein kinase in filamentous fungi. (2005) Eukaryotic Cell 4:1820-1828.

Iwamoto T, Mise K, Takeda A, Okinaka Y, Mori K, Arimoto M, Okuno T,and Nakai, T. Characterization of Striped jack nervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B2. (2005) Journal of General Virology 86: 2807-2816.

Takeuchi S, Hamada H, Toyoda K, Suzuki K, Kiba A, Hikichi Y,and Okuno T. Discrimination between tobamoviruses and their pathotype for L-gene-mediated resistance in green pepper (Capsicum anuum L.) by reverse transcription-polymerase chain reaction. (2005) Journal of General Plant Pathology 71: 60-67.

Hamada H, Takeuchi S, Kiba A, Tsuda S, Suzuki K, Hikichi Y, and Okuno T. Timing and extent of hypersensitive response are critical to restrict local and systemic spread of Pepper mild mottle virus in pepper containing the L3 gene. (2005) Journal of General Plant Pathology 71: 90-94.

Watanabe T, Takeda A, Mise K, Okuno T, Suzuki T, Minami N,and Imai H. Stage-specific expression of microRNAs during Xenopus development. (2005) FEBS Letters 579: 318-324.

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Laboratory of Plant Pathology, Graduate School of Agriculture,
Kyoto University
Kyoto 606-8502, Japan
TEL: +81-75-753-6131
FAX: +81-75-753-6133